참고 기사 “유전자 가위, ‘표적 벗어난 변이’ 예상보다 많이 발생”   http://scienceon.hani.co.kr/522774

Unexpected mutations after CRISPR–Cas9 editing in vivo

• Kellie A Schaefer,
• Wen-Hsuan Wu,
• Diana F Colgan,
• Stephen H Tsang,
• Alexander G Bassuk
• & Vinit B Mahajan

Nature Methods

14,

547–548

(2017)

doi:10.1038/nmeth.4293

Published online

30 May 2017

CRISPR–Cas9 editing shows promise for correcting disease-causing mutations. For example, in a recent study we used CRISPR-Cas9 for sight restoration in blind rd1 mice by correcting a mutation in the Pde6b gene¹. However, concerns persist regarding secondary mutations in regions not targeted by the single guide RNA (sgRNA)².…