인간 배아줄기세포 배양에서 마이코플라즈마 오염 시 제거를 위한 연구
= Elimination of Mycoplasma Contamination in Human Embryonic Stem Cells Culture

제어번호 100246025
저자명 윤보애 ( Bo Ae Yoo )  ,백진아 ( Jin Ah Baek )  ,설혜원 ( Hye Won Seol )  ,정주원 ( Ju Won Jung )  ,

           오선경 ( Sun Kyung Oh )  ,최영민 ( Young Min Choi )
학술지명 人口醫學硏究論集
권호사항 Vol.27 No.- [2014] 
발행처 서울대학교 인구의학연구소 
자료유형 학술저널
수록면 82-87(6쪽)
언어 Korean
발행년도 2014년
판매처 한국학술정보

 
초록
Mycoplasma is one of the smallest and simplest prokaryotes. Mycoplasma contamination during cell culture is widespread, and the use of contaminated cells compromises almost all aspects of cell physiology and consequently the results and conclusions from numerous experiments. In this study, we tried to eliminate the mtcoplasma from mtcoplasma contaminated cases during human embryonic stem cell(hESC) culture. hESC lines (SNUhES4, SNUhE31, H1) were cultured with three diffetent antimycoplasma antibiotics (Moxifloxacin+Gentamicin, Plasmocin, Plasmocure) for 4~5 passges, We observed morphologies, rete of attachment and differentiation of hESCs. Also, we confirmed relative treatment efficiency (eliminated, resistant, dead) of antimtcoplasma antibiotics. Three weeks after Plasmocure treatment, high attachment tate and mycoplasma free condition were well maintained. Relative treatment efficiency of Plasmocure showed eliminated 88.9%, resisrant 11.1%, dead 0%. Among the tested antimycoplasma antibiotics, Plasmocure was a most effective antimycoplasma antibiotic to eliminate mycoplasma from contaminated cell culture cases.

주제어
Mycoplasma  ,contamination  ,antimycoplasma antibiotics