원저 : 돼지생식기호흡기증후군바이러스(PRRSV)의 전장 유전체염기서열(whole-genome sequencing) 분석을 위한 차세대 염기서열 분석법의 활용
= Original Article : Application of next generation sequencing (NGS) system for whole-genome sequencing of porcine reproductive and respiratory syndrome virus (PRRSV)

 
제어번호 101832724
저자명 문성현 ( Sung-hyun Moon )  , 아미나카툰 ( Amina Khatun )  , 김원일 ( Won-il Kim )  ,

           후세인엠디묵터 ( Md Mukter Hossain )  , 오연수 ( Yeon Su Oh )  , 조호성 ( Ho-seong Cho ) 
학술지명 韓國家畜衛生學會誌
권호사항 Vol.39 No.1 [2016] 
발행처 한국가축위생학회 
자료유형 학술저널
수록면 41-49(9쪽)
언어 Korean
발행년도 2016년
등재정보 KCI등재
판매처 한국학술정보

 
초록
In the present study, fast and robust methods for the next generation sequencing (NGS) were developed for analysis of PRRSV full genome sequences, which is a positive sensed RNA virus with a high degree of genetic variability among isolates. Two strains of PRRSVs (VR2332 and VR2332-R) which have been maintained in our laboratory were used to validate our methods and to compare with the sequence registered in GenBank (GenBank accession no. EF536003). The results suggested that both of strains had 100% coverage with the reference; the VR2332 had the coverage depth from minimum 3 to maximum 23,012, for the VR2332-R from minimum 3 to maximum 41,348, and 22,712 as an average depth. Genomic data produced from the massive sequencing capacities of the NGS have enabled the study of PRRSV at an unprecedented rate and details. Unlike conventional sequence methods which require the knowledge of conserved regions, the NGS allows de novo assembly of the full viral genomes. Therefore, our results suggested that these methods using the NGS massively facilitate the generation of more full genome PRRSV sequences locally as well as nationally in regard of saving time and cost.

주제어
Next Generation Sequencing  , NGS  , PRRSV  , Whole-genome sequencing