학술지명

韓國受精卵移植學會誌                             

학술지 이력

연월일	이력구분	이력상세	등재구분
2017	평가예정	계속평가 신청대상(등재유지)
2013-01-01	평가	등재학술지 유지(등재유지)
2010-01-01	평가	등재학술지 유지(등재유지)
2007-12-05	학술지명변경	한글명 : 한국수정란이식학회지 외국어명 : Korean Journal of Embryo Transfer
2007-01-01	평가	등재학술지 선정(등재후보2차)
2006-02-13	학술지명변경	한글명 : 한국수정란이식학회지 외국어명 : Korean Journal of Embryo Transfer
2006-01-01	평가	등재후보 1차 PASS(등재후보1차)
2005-06-07	학술지명변경	한글명 : 한국수정란이식학회지 외국어명 : Korean Journal of Embryo Transfer
2005-01-01	평가	등재후보 1차 FAIL(등재후보1차)
2003-07-01	평가	등재후보학술지 선정(신규평가)

학술지 인용정보

기준년도	WOS-KCI 통합IF(2년)	KCI IF(2년)	KCI IF(3년)
2014	0.11	0.11	0.11
KCI IF(4년)	KCI IF(5년)	중심성지수(3년)	즉시성지수
0.12	0.11	0.33	0.04

Many pronuclear stage eggs were used to generate transgenic mice (Tg) by microinjection. In this study, we used in vitro fertilized mouse eggs, followed by ultrarapid freezing to establish a simple procedure for production of Tg mice. We produced in vitro fertilized mouse eggs and cryopreserved them by ultrarapid freezing method. A total of 139 cryopreserved-thawed pronuclear eggs, of which 101 (72.6%) were survived following microinjection of chicken b-actin promoter-driven firefly improved luciferase cDNA (β-act/luc+) and were transferred into 5 recipients. All recipients became pregnant and gave birth to a total of 15 (14.8%) pups. As a control, same DNA construction (β- act/luc+) was also injected into 450 in vitro fertilized eggs, of which 338 (75.1%) were survived and then were transferred into 14 recipients. Eleven (78%) mice became pregnant and littered a total of 54 (19.1%) pups. Southern blotting analysis of Tg mice indicated that one (1/15, 6.6%) and three (3/54, 5.5%) transgenic mice were production from cryopreserved and in vitro fertilized eggs, respectively. All Tg mice produced from both eggs showed the expression of improved luciferase gene. These results indicated that efficiency of produced of Tg mice from cryopreserved eggs was comparable to that from in vitro fertilized eggs. Furthermore, it is suggested that microinjection of transgene into in vitro fertilized eggs cryopreserved by ultrarapid freezing is an easy and conveniently method for production of Tg mice.