법과학적 활용을 위한 식물 유전자검사 시험법 연구

 
제어번호 101839649
저자명 한시내  ,박은영  ,이혜진  ,안희중
학술지명 한국법과학회지(Korean journal of forensic science)
권호사항 Vol.17 No.1 [2016] 
발행처 한국법과학회 
발행처URL http://www.ksfs.org
자료유형 학술저널
수록면 32-36(5쪽)
언어 Korean
발행년도 2016년
판매처 학술교육원

 
초록
DNA analysis results from body fluid remaining at the crime scenes has adopted by the important evidences for solving criminal cases. Recently, plant material evidences has become increasingly important for solving criminal cases. In this study, we has established the optimized method for plant DNA analysis to aid in forensic cases. We used MagNA Lyser that disrupt samples and select psbA-trnH spacer region to discriminate plant species. Among Taq polymerase, BD Advantage 2 polymerase mix and i-TaqTM plus DNA polymerase showed successful results in DNA amplification efficiency at 1.0 ng/uL of DNA template, however TaKaRa Taq polymerase and AmpliTaq Gold polymerase showed no PCR products. Especially BD Advantage 2 polymerase mix showed successful results in DNA amplification and sequencing below 0.01 ng/uL DNA template concentration. However it was necessary to use more than two loci region in plastid genomes for further evaluation to increase discrimination power.

 
목차
I. 서론
II. 재료 및 방법
1. 시료 준비
2. DNA 분리
3. 유전자 증폭
4. 염기서열분석 및 식물 종 확인
III. 결과 및 고찰
IV. 결론
V. 참고문헌

주제어
Plant DNA identification  , psbA-trnH  , DNA polymerase