임상으로부터 분리된 Candida albicans의 MLST를 통한 유전적 변이 분석

 
저자 김희진
형태사항 69 ; 26 cm
일반주기 지도교수: 김영권
학위논문사항 학위논문(석사)-- 건양대학교 : 보건학과(임상병리학) 2016. 2
발행국 충청남도
언어 한국어
출판년 2016
소장기관 건양대학교 명곡도서관

초록
The MLST(multilocus sequence typing) of Candida albicans is based on the analysis of molecular biological identification. This analysis based on DNA sequencing is to compare the difference of base sequence in bacteria species to identify the genetic variations in the same species. In this study, a MLST analysis were performed on clinically isolated 40 strains C. albicans isolated from hospital located in Korea in order to identify the genetic characteristics by sequence variation as well as phylogenetic relation of strains collected from different sources. The general variations found in 7 different housekeeping genes of C. albicans collected from urine & sputum, peripheral blood, central line blood, other specimens were analyzed. As a result, a total of 37 polymorphic sites were found in ACC1 (4), VPS13 (7), GLN4 (4), ADP1 (8), RPN2 (5), PMA1 (4) and SYA1 (5). Three clusters including the normal flora in phylogenetic tree, peripheral blood and central line blood were created. Consequently, the genetically similarity as well as the potential relevance were found in the strains collected from same specimen sources. MLST analysis is thought to be useful to determine the variation of species such as genetically various mutations. I believe that continuous accumulation of data on the genetic mutations of C. albicans related to phylogenetic aspects will help develop the antibiotics of new genes and establish epidemiological surveillance system for studying infectious diseases of the mechanism.

 
목차
제1장 서 론
제2장 연구대상 및 방법
1. 재료
2. Candida 속의 표현형 분석 
3. 분자생물학적 특성 분석
제3장 결 과
제1절 균주 확인 동정
제2절 C. albicans 유전자 확인
1. genomic DNA 추출 및 D1/D2 region의 증폭
2. housekeeping gene 및 염기서열 확인
제3절 임상분리 C. albicans의 housekeeping gene 분석
1. 각 housekeeping gene의 염기서열 변이 분석
1) ACC1 유전자 변이
2) VPS13 유전자 변이
3) GLN4 유전자 변이
4) ADP1 유전자 변이
5) RPN2 유전자 변이
6) PMA1 유전자 변이
7) SYA1 유전자 변이
8) 변이율 확인
2. C. albicans 유전자의 MLST 분석

제4장 고 찰

제5장 결 론

참고문헌
요약문
감사의 글

주제어
임상, Candida albicans, MLST, 유전적 변이분석