물리적 인장 자극에 의한 줄기세포의 분화에 동반되는 미토콘드리아의 특성 변화에 관한 고찰
= Original Article : Changes in Mitochondrial Properties during Stem Cell Differentiation induced by Mechanical Stretching

 
제어번호 100282440
저자명 신지원 ( Ji Won Shin )  ,김혜림 ( Hye Lim Kim )  ,강윤경 ( Yun Gyeong Kang )  ,박소희 ( So Hee Park )  ,

           김영미 ( Young Mi Kim )  ,신정욱 ( Jung Woog Shin ) 
학술지명 조직공학과 재생의학(Tissue Engineering and Regenerative Medicine)
권호사항 Vol.12 No.1 [2015] 
발행처 한국조직공학과 재생의학회(Korean Tissue Engineering & Regenerative Medicine Society) 
자료유형 학술저널
수록면 60-65(6쪽)
언어 Korean
발행년도 2015년
등재정보 KCI등재
판매처 한국학술정보

 
초록
Recent studies have confirmed that the direction of stem cell differentiation can be modulated by physical stimuli even without biochemical growth factors. This implies stem cells attribute their differentiation to mechanical signaling directly or indirectly. However, the role or changes in mitochondria, one of the major energy sources in a cell, during differentiation have not been investigated. This study applied mechanical stretching of two different magnitudes (3% and 10%) to human mesenchymal stem cells without any relevant growth factors. We confirmed differentiation of stem cells into two different directions: osteogenic- and smooth muscle-like cells. Along with these results we found the expression of NOX4 was increased with stretching magnitude. As the expression of NOX4 is closely related to mitochondrial biogenesis and morphology various markers were also investigated. The expressions of the markers in relation to biogenesis (PGC-1alpha, TFA) and fusion (MFN1, MFN2, OPA1) were increased with stretching magnitude. However, the expression of Drp1 was observable with stretching of 3%. This study showed that the magnitude of physical stimulation affects the expression of NOX4, which has been known to be closely related to the biogenesis and morphological changes of mitochondria and stem cell differentiation. Therefore, further study of NOX4 due to physical stimulation for the control of stem cell differentiation accompanied by mitochondria functions is worth to be performed.

주제어
mesenchymal stem cells  ,stem cell differentiation  ,mechanical stretch  ,mitochondrial biogenesis  ,mitochondrial morpholog